Testing Sample Extrusion Using a High-Speed Camera Setup
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Results: Extrusion of Protein Microcrystals from a High Viscosity Micro-extrusion Injector
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Conclusion
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This protocol has been designed to ensure that problems frequently encountered during time-resolved serial crystallographic experiments won't compromise data quality. One major advantage of this method is its adaptability to individual crystal sys
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The success of a time-resolved serial femtosecond crystallography experiment is dependent on efficient sample delivery. Here, we describe protocols to optimize the extrusion of bacteriorhodopsin microcrystals from a high viscosity micro-extrusion injector. The methodology relies on sample homogenization with a novel three-way coupler and visualization with a high-speed camera.