Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
Transplantation of Syngeneic Breast Tumors into Mouse Hosts with Fluorescent Macrophages
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Preparation of Mouse for Imaging
4:25
Evaluation of Extravascular Dextran Using Fixed Tissue Analysis
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Results: Visualization of Vasculature in Healthy and Diseased Tissues and TMEM-Mediated Vascular Permeability
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Conclusion
Transcribir
These complementary techniques evaluate and measure the permeability of tumor vasculature using high-molecular weight Dextran. The fixed-tissue protocol offers larger tumor coverage, is high throughput, and does not require multiphoton imaging equ
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We describe two methods for assessing transient vascular permeability associated with tumor microenvironment of metastasis (TMEM) doorway function and cancer cell intravasation using intravenous injection of high-molecular weight (155 kDa) dextran in mice. The methods include intravital imaging in live animals and fixed tissue analysis using immunofluorescence.