Pregnancy Confirmation and E10.5 Embryo Harvest and Slice Preparation
4:15
Results: Representative Ex Vivo Oculomotor Slice Culture Development and Pitfalls
5:30
Conclusion
Transcribir
This protocol allows us to identify axon guidance pathways active in the oculomotor nerve and to assess their roles at different points along the nerve trajectory in real time. This technique preserves the local environments through which axons tr
Inicia sesión o comienza tu prueba gratuita para acceder a este contenido
An ex vivo slice assay allows oculomotor nerve outgrowth to be imaged in real time. Slices are generated by embedding E10.5 IslMN:GFP embryos in agarose, slicing on a vibratome, and growing in a stage-top incubator. The role of axon guidance pathways is assessed by adding inhibitors to the culture media.