Results: Representative B. bacteriovorus Life Cycle Captured by Time-Lapse Fluorescence
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Conclusion
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Live-Cell Bdellovibrio bacteriovorus imaging has been challenging due to the complex life cycle of these predatory bacteria. Our protocol allows monitoring of the stages of complete life cycle in real time. Although our protocol is based on a spec
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Presented here is a protocol that describes monitoring of the complete life cycle of predatory bacterium Bdellovibrio bacteriovorus using time-lapse fluorescence microscopy in combination with an agarose pad and cell-imaging dishes.