Treatment of RPE Cells with Growth Factors and H2O2
4:33
Glutathione Assay
6:00
Cytotoxicity Assay and Microscopic Analysis
7:24
Results: Representative Oxidative Stress Induction and Analysis
9:10
Conclusion
Transcripción
The hydrogen peroxide model is a simple and effective approach for evaluating the antioxidant effects of factors that may be useful in treating age-related macular degeneration or similar neurodegenerative diseases. The oxidant agent, hydrogen per
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We present a protocol for the development and use ofan oxidative stress-model by treating retinal pigment epithelial cells with H2O2, analyzing cell morphology, viability, density, glutathione, and UCP-2 level. It is a useful model to investigate the antioxidant effect of proteins secreted by transposon-transfected cells to treat neuroretinal degeneration.