KCl-mediated Depolarization of Isolated Synaptic Terminals
3:21
Fixation and Phalloidin Staining of Samples
4:40
Fluorometric Analysis and Light Scattering
5:20
Data Analysis
6:19
Conclusion
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Actin is the major component of cytoskeleton and critically regulates several aspects of cellular morphology and physiology, including in neurons. Actin occurs in equilibrium between its two forms, monomeric globular G-actin or polymeric filamento
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We report a simple, time-efficient and high-throughput fluorescence spectroscopy-based assay for the quantification of actin filaments in ex vivo biological samples from brain tissues of rodents and human subjects.