Frozen Tissue Dissociation and Ultracentrifugation
2:13
Fluorescence-Activated Nuclei Sorting (FANS)
4:32
Downstream Molecular Analyses
5:58
Results: Analysis of Human Astrocyte Populations after FANS
7:40
Conclusion
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This protocol can help scientists who are interested in studying the molecular function of human astrocytes in their native niche. The main advantage of this technique is that it allows for the isolation and enrichment of a specific cell type, adu
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We have developed a method that enriches for and isolates human astrocyte populations from fresh-frozen tissue for use in downstream molecular analyses.