Performing the Craniotomy and Exposing the Somatomotor Cortex
2:21
Injecting Virus into the Somatomotor Cortex
3:00
Creating a Spinal Window for Precise Spinal Cord Injections
5:31
Direct Injections into the Spinal Cord Targeting Axon Terminals
6:33
Results: Transduction of Neurons in the Motor Cortex
6:52
Conclusion
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This is the first protocol demonstrating the successful co-infection of the corticospinal tract in neonatal rats. It provides an accurate blueprint to investigate how individual subpopulations of cells in young mammals contribute to the recovery f
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This protocol demonstrates a novel method for applying gene therapies to subpopulations of cells in neonatal rats at postnatal ages 5-10 days by injecting an anterograde chemogenetic modifier into the somatomotor cortex and a retrogradely transportable Cre recombinase into the cervical spinal cord.