Fluorescence Imaging of Gcamp6m Calcium Transients
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Image Analysis
6:23
Results: Real-Time Monitoring of the Dynamics of Presynaptic Calcium Influx and Synaptic Vesicle Membrane Fusion in In Vitro Cultured Neurons
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Conclusion
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These methods enable quick assessment as to whether an experimental manipulation, disease causative protein, or RNA can impact synaptic processes, and whether therapeutic compounds can restore function. These techniques provide a reliable readout
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Two related methods are described to visualize subcellular events required for synaptic transmission. These protocols enable the real-time monitoring of the dynamics of presynaptic calcium influx and synaptic vesicle membrane fusion using live-cell imaging of in vitro cultured neurons.