Isolating Primary Human Smooth Muscle Cells (SMCs) from Aortic Biopsies
2:40
Isolating Primary Dermal Fibroblasts from Skin Biopsies
4:25
Measuring Contraction
6:00
Results: Measurement of Aortic SMC Contraction upon Ionomycin Stimulation and SMC Marker Expression
7:51
Conclusion
Transcribir
This method allows researchers to study the cell contraction of multiple patient cell line simultaneously. It allows us to study diseases quicker and more efficiently. This technique allows us to quantify cell contraction in real time.
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This paper describes an explant culture-based method for the isolation and culturing of primary, patient-specific human aortic smooth muscle cells and dermal fibroblasts. Furthermore, a novel method is presented for measuring cell contraction and subsequent analysis, which can be used to study patient-specific differences in these cells.