Debris Removal and Magnetic Separation of Microglial Cells
3:26
Results: Gating Strategy Used in Flow Cytometry Analysis Before and After MACS and Gating Strategy for Living/Dead Single Cells After MACS
4:27
Conclusion
Transcripción
This protocol helps to isolate microglia from the microdissected demyelination lesions of the adult mouse brain to study the microglial characteristics in vivo. This technique saves time and has lower requirements for both experimenters and equipm
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Here, we present a protocol to isolate and purify primary microglia in animal models of demyelinating diseases, utilizing columnar magnetic-activated cell sorting.