S'identifier

Illinois Institute of Technology

3 ARTICLES PUBLISHED IN JoVE

Medicine

Generation of Alginate Microspheres for Biomedical Applications

Omaditya Khanna¹, Jeffery C. Larson², Monica L. Moya³, Emmanuel C. Opara⁴, Eric M. Brey^2,5

¹Department of Chemical and Biological Engineering, Illinois Institute of Technology, ²Department of Biomedical Engineering, Illinois Institute of Technology, ³Department of Biomedical Engineering, University of California at Irvine, ⁴Wake Forest Institute for Regenerative Medicine and Department of Biomedical Engineering, Wake Forest University Health Sciences, ⁵Research Service, Hines Veterans Administration Hospital

In the following sections, we outline procedures for the preparation of alginate microspheres for use in biomedical applications. We specifically illustrate a technique for creating multilayered alginate microspheres for the dual purpose of cell and protein encapsulation as a potential treatment for type 1 diabetes.

Cancer Research

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Ali Ansari¹, Reema Patel², Kinsey Schultheis¹, Vesna Naumovski³, P. I. Imoukhuede¹

¹Department of Bioengineering, University of Illinois at Urbana-Champaign, ²Department of Liberal Arts & Sciences, University of Illinois at Urbana-Champaign, ³Department of Biomedical Engineering, Illinois Institute of Technology

This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.

Education

X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

Weikang Ma¹, Thomas C. Irving¹

¹BioCAT, Dept. of Biological Sciences, Illinois Institute of Technology

We present detailed protocols for performing small-angle X-ray diffraction experiments using intact mouse skeletal muscles. With the wide availability of transgenic mouse models for human diseases, this experimental platform can form a useful test bed for elucidating the structural basis of genetic muscle diseases