Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological ConditionsNicolas Melis *1, Bhagawat Subramanian *1,2, Desu Chen 1, Roberto Weigert 1
1Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, 2Lineberger Comprehensive Cancer Center, UNC-Chapel Hill
Neutrophil migration relies on the rapid and continuous remodeling of the plasma membrane in response to chemoattractant and its interactions with the extracellular microenvironment. Described herein is a procedure based on Intravital Subcellular Microscopy to investigate the dynamic of membrane remodeling in neutrophils injected in the ear of anesthetized mice.
