Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeriCharlotte Philippe 1,2, Arnout F. Gregoir 1, Eli S. J. Thoré 1, Gudrun De Boeck 2, Luc Brendonck 1,3, Tom Pinceel 1,4
1Animal Ecology, Global Change and Sustainable Development, University of Leuven, 2Systemic Physiological and Ecotoxicological Research, University of Antwerp, 3Water Research Group, Unit for Environmental Sciences and Management, North-West University, 4Centre for Environmental Management, University of the Free State
In this work, we describe an acute, chronic and multigenerational bioassay to study the effects of single and combined stressors on the Turquoise killifish Nothobranchius furzeri. This protocol is designed to study life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.
