California NanoSystems Institute, University of California

2 ARTICLES PUBLISHED IN JoVE

Deriving De Novo Atomic Models by Cryo Electron Microscopy

Peng Ge¹, Nicole Poweleit^1,2, Robert P Gunsalus^2,3, Hong Zhou^1,2

¹Electron Imaging Center for Nanomachines (EICN), California NanoSystems Institute, University of California, ²Immunology and Molecular Genetics, University of California, ³UCLA Institute of Genomics and Proteomics

Cryo electron microscopy (cryoEM) can be employed to derive de novo atomic models of macromolecular complexes in solution. The steps involved in high resolution cryoEM of biological molecules, from image recording, to data processing, to atomic modeling based on the resulting cryoEM density map, are illustrated.

Engineering

Interactive and Visualized Online Experimentation System for Engineering Education and Research

Zhongcheng Lei¹, Hong Zhou¹, Shengwang Ye¹, Wenshan Hu¹, Guo-Ping Liu¹, Zijie Wei¹

¹Department of Artificial Intelligence and Automation, Wuhan University

This work describes an online experimentation system that provides visualized experiments, including the visualization of theories, concepts, and formulas, visualizing the experimental process with three-dimensional (3-D) virtual test rigs, and visualizing the control and monitoring system using widgets such as charts and cameras.