Chinese Academy of Medical Science and Peking Union Medical College

2 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation and Identification of Porcine Bone Marrow Mesenchymal Stem Cells and their Derived Extracellular Vesicles

Wenyang Jiang¹, Yongchun Cui², Xiaosong Li¹, Yuyan Xiong¹, Guangxin Yue², Yuejin Yang³

¹State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Science and Peking Union Medical College, ²State Key Laboratory of Cardiovascular Disease, Center for Cardiovascular Experimental Study and Evaluation, National Center for Cardiovascular Diseases, Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, Animal Experimental Center, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, ³State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Science and Peking Union Medical College

This article elaborates a method to isolate and identify porcine bone marrow mesenchymal stem cells (pBM-MSCs) and extracellular vesicles (EVs) derived from them, providing a methodological basis for the pre-clinical evaluation of transplantation efficacy of BM-MSCs and their derived EVs.

Biology

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

Dingyun Song^1,2, Yicong Chen^1,3, Xin Wang^1,4, Xueying Chen^1,2, Shuwei Gao^1,3, Wenxiu Xu^1,3, Shengnan Yang⁵, Zai Wang⁶, Liang Peng⁷, Huaping Dai^1,2

¹Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Diseases, Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, ²Graduate School of Peking Union Medical College, Chinese Academy of Medical Science and Peking Union Medical College, ³Capital Medical University, ⁴Beijing University of Chinese Medicine, ⁵Tianjin Chest Hospital, ⁶Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, ⁷Beijing Key Laboratory for Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital

Various animal models of pulmonary fibrosis have been established using bleomycin to clarify the pathogenesis of pulmonary fibrosis and find new drug targets. However, most pulmonary fibrosis models targeting lung tissue have uneven drug administration. Here, we propose a model of uniform pulmonary fibrosis induced by nasal bleomycin nebulization.