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Graduate School of Medicine, Kyoto University

5 ARTICLES PUBLISHED IN JoVE

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Biology

Light/dark Transition Test for Mice
Keizo Takao 1, Tsuyoshi Miyakawa 1
1Genetic Engineering and Functional Genomics Unit, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University

The light/dark transition test is one of the most widely used tests to measure anxiety-like behavior in mice. Here, we present a movie that shows detailed procedures on how we conduct the test.

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Biology

Elevated Plus Maze for Mice
Munekazu Komada 1, Keizo Takao 1,2, Tsuyoshi Miyakawa 2
1Genetic Engineering and Functional Genomics Unit, Frontier Technology Center, Graduate School of Medicine, Kyoto University, 2Institute for Comprehensive Medical Science Division of Systems Medicine, Fujita Health University

The elevated plus maze test is one of the most widely used tests for measuring anxiety-like behavior in mice. Here, we present a movie showing the detailed procedures for conducting the test.

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Biology

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
Hideo Hagihara 1,2, Keiko Toyama 1,2, Nobuyuki Yamasaki 1,3, Tsuyoshi Miyakawa 1,2,4,5
1Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), 2Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, 3Department of Psychiatry, Graduate School of Medicine, Kyoto University, 4Genetic Engineering and Functional Genomics Group, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, 5Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

A dissection technique for removal of the dentate gyrus from adult mouse under a stereomicroscope was demonstrated in this video-recorded protocol.

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Neuroscience

T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice
Hirotaka Shoji 1,2, Hideo Hagihara 1, Keizo Takao 3, Satoko Hattori 1,2,3, Tsuyoshi Miyakawa 1,2,3
1Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, 2Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), 3Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

This article presents the protocol of T-maze tests using a modified automated apparatus for assessing the learning and memory functions in mice.

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Behavior

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice
Hirotaka Shoji 1,2, Keizo Takao 2,3, Satoko Hattori 1,2, Tsuyoshi Miyakawa 1,2,3
1Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, 2Japan Science and Technology Agency, Core Research for Evolutionary Science and Technology (CREST), 3Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, National Institutes of Natural Sciences

This article presents a protocol for a contextual and cued fear conditioning test using a video analyzing system to assess fear learning and memory in mice.

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