Hangzhou Normal University

3 ARTICLES PUBLISHED IN JoVE

Education

Facile Preparation of (2Z,4E)-Dienamides by the Olefination of Electron-deficient Alkenes with Allyl Acetate

Liyuan Ding¹, Chunbing Yu¹, Zhenqiang Zhao², Feifei Li¹, Jian Zhang¹, Guofu Zhong¹

¹College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, ²Shijiazhuang Mechanized Infantry Academy

The ruthenium-catalyzed olefination of electron-deficient alkenes with allyl acetate is described here. By using aminocarbonyl as a directing group, this external oxidant-free protocol has high efficiency and good stereo- and regioselectivity, opening a novel synthetic route to (Z,E)-butadiene skeletons.

Chemistry

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Yang Wu^*1, Wei Zhang^*2, Jian Zhang², Zhi-Xiang Mao³, Li Ding², Hao Li³, Rong Ma¹, Jin-Hai Tang²

¹Research Center of Clinical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Nanjing Medical University Affiliated Cancer Hospital, ²Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, ³School of Clinical Medicine, Xuzhou Medical University

This work presents the preparation of methionine functionalized biocompatible block copolymers (mBG) via the reversible addition-fragmentation chain transfer (RAFT) method. The plasmid DNA complexing ability of the obtained mBG and their transfection efficiency were also investigated. The RAFT method is very beneficial for polymerizing monomers containing special functional groups.

Medicine

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Limei Wang¹, Jianmin Guo¹, Xiaoman Zhong¹, Jian Zhang², Yali Sheng¹, Qiwen Lai¹, Hui Song¹, Wei Yang¹

¹Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, TCM Non-clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Engineering Research Center for Innovative Drug Evaluation and Research, ²Department of Biomedical Engineering, School of Basic Medical Sciences, the Sixth Affiliated Hospital of Guangzhou Medical University

The present protocol describes a simple and efficient method for collecting blood from the subclavian vein in rats. It enables rapid, timely, and easily identifiable sampling without anesthesia and obtains high-quality blood through repetitive sample collection.