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Ningxia medical university

5 ARTICLES PUBLISHED IN JoVE

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Medicine

Observation of the Systolic Function of Isolated Right Atria from Guinea Pigs
Jiayuan Gu *1, Zhengjun Zhang *2, Jonnea Japhet Tibenda *1, Lin Yan 3, Yafei Zhu 4, Qipeng Zhao 1,5,6
1School of Pharmacy, Ningxia Medical University, 2Department of Cardiology, General Hospital of Ningxia Medical University, 3School of Basic Medical Sciences, Ningxia Medical University, 4College of Nursing, Ningxia Medical University, 5Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education Ningxia Medical University, 6Ningxia Regional Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Regional High Incidence Disease

The present protocol describes an efficient method for screening drugs that enhance myocardial contractility using isolated right atria from guinea pigs.

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Neuroscience

Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection
Fuhan Fan 1, Hong Jiang 1, Ya Hou 1, Yi Zhang 1,2,3, Qipeng Zhao 4, Yong Zeng 1,2, Xianli Meng 1,2,5, Xiaobo Wang 2
1School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine, 2Meishan Hospital of Chengdu University of Traditional Chinese Medicine, 3School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, 4School of Pharmacy, Ningxia Medical University, 5Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine

This protocol describes a dependable and efficient in vitro model of the brain blood barrier. The method uses mouse cerebral vascular endothelial cells bEnd.3 and measures transmembrane electrical resistance.

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Medicine

Bioinformatics-based Analyses and B/Th Cell Epitope Prediction of Mugwort Pollen Allergen Art v 1 Protein
Jin Hu *1,2,3, Xiangyu Zhou *4, Yikun Cao *4, Xiuli Cheng *5, Na Li 4, Bing Hua 4, Hongwan Dang 1,3
1School of Basic Medicine, Ningxia Medical University, 2Preparation Center, General Hospital of Ningxia Medical University, 3Department of Pharmacy, General Hospital of Ningxia Medical University, 4Institute of Neurological Diseases, Shizuishan First People's Hospital, 5Department of Pharmacy, People's Hospital of Ningxia Hui Autonomous Region

Here, we present a protocol to perform bioinformatics correlation analysis to predict the physicochemical properties, secondary structure, and B and T helper cell epitopes of mugwort pollen main allergen Art v 1 protein to provide a theoretical basis for the subsequent development of artemisia pollen allergic disease vaccine and disease treatment.

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Neuroscience

Role of CD4 T Cell in Relapsing-Remitting Experimental Autoimmune Encephalomyelitis
Jin Hu *1,2, Xiangyu Zhou *3, Yikun Cao *3, Huili Tian *4, Na Li 3, Xiuli Cheng 5, Xiaoying Yang 6, Hongwan Dang 1,6
1School of Basic Medicine, Ningxia Medical University, 2Preparation Center, General Hospital of Ningxia Medical University, 3Institute of Neurological Diseases, First People's Hospital of Shizuishan, 4Department of Neurology, Xianyang Hospital of Yan’an University, 5Department of Pharmacy, People’s Hospital of Ningxia Hui Autonomous Region, 6Department of Pharmacy, General Hospital of Ningxia Medical University

An experimental autoimmune encephalomyelitis (EAE) model in mice was employed to investigate the role of CD4 T cells in the initial and relapse of EAE from the perspective of the activation phase and immune effect function.

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Medicine

Sampling of N-methyl-N-nitrosourea-induced Rat's Retina Damage and Multi-index Evaluation of Pathological Changes
Yafei Zhu *1, Xinlin Zhao *1, Tibenda Jonnea Japhet 1, Jun Chen 2, Qipeng Zhao 1
1School of Nursing, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, 2Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine

This protocol describes a procedure for rat retinal sampling and utilizing hematoxylin-eosin staining, TUNEL assay, and Western blot to detect pathological changes and apoptosis in the retina after intraperitoneal injection of N-Methyl-N-Nitrosourea.

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