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Wellcome Trust Sanger Institute, Wellcome Genome Campus

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Mercedes Pardo 1, Daniel Bode 1, Lu Yu 1, Jyoti S. Choudhary 1
1Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute

Here we present protocols for affinity purification of protein complexes and their separation by blue native PAGE, followed by protein correlation profiling using label free quantitative mass spectrometry. This method is useful to resolve interactomes into distinct protein complexes.

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Genetics

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
Christoph N. Schlaffner 1,2,3, Georg J. Pirklbauer 2, Andreas Bender 3, Judith A.J. Steen 1, Jyoti S. Choudhary 2,4
1Department of Neurobiology, F. M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, 2Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Wellcome Genome Campus, 3Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, 4Functional Proteomics Group, Chester Beatty Laboratories, Institute of Cancer Research

Here we present the proteogenomic tool PoGo and protocols for fast, quantitative, post-translational modification and variant enabled mapping of peptides identified through mass spectrometry onto reference genomes. This tool is of use to integrate and visualize proteogenomic and personal proteomic studies interfacing with orthogonal genomics data.

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