xi'an jiaotong university

8 ARTICLES PUBLISHED IN JoVE

Cancer Research

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Rui Xue Zhang¹, Tian Zhang¹, King Chen¹, Ji Cheng¹, Paris Lai¹, Andrew M. Rauth², K. Sandy Pang¹, Xiao Yu Wu¹

¹Department of Pharmaceutical Sciences, University of Toronto, ²Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute, University Health Network

This protocol describes an efficient and convenient analytical process of sample extraction and simultaneous determination of multiple drugs, doxorubicin (DOX), mitomycin C (MMC) and a cardio-toxic DOX metabolite, doxorubicinol (DOXol), in the biological samples from a preclinical breast tumor model treated with nanoparticle formulations of synergistic drug combination.

Medicine

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch

Yaguang Li¹, Junhong Liu^2,3, Gaixia Liu¹, Zhenhong Pan^2,3, Mingxia Zhang¹, Yao Ma⁴, Qingxia Wei^1,5, Hongping Xia^1,6, Rui Xue Zhang^1,2, Junjun She¹

¹Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, ²Institute of Medical Research, Northwestern Polytechnical University, ³School of Life Sciences, Northwestern Polytechnical University, ⁴Department of Medicine, Xi'an Jiaotong University, ⁵Princess Margaret Cancer Center, University of Health Network, ⁶Department of Pathology, Nanjing Medical University

The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease.

Neuroscience

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

Rui Yang^1,2,3, Vann Bennett^2,3

¹School of Basic Medical Sciences, Xi'an Jiaotong University, ²Department of Cell Biology, Duke University, ³Department of Biochemistry, Duke University

Here, we described a protocol to quantitatively study the assembly and structure of the axon initial segments (AIS) of hippocampal neurons that lack pre-assembled AIS due to the absence of a giant ankyrin-G.

Neuroscience

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

YangDong Wang^1,2,3,4, DanYang Zhu⁵, BaoYue Liu^1,2,3,6, Kiryl D. Piatkevich^1,2,3

¹School of Life Sciences, Westlake University, ²Westlake Laboratory of Life Sciences and Biomedicine, ³Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, ⁴School of Basic Medical Sciences, Xi'an JiaoTong University, ⁵Office of Public Affairs, Westlake University, ⁶School of Pharmaceutical Sciences, Jilin University

This article demonstrates the preparation of a custom-made imaging window supplemented with infusion cannula and its implantation onto the CA1 region of the hippocampus in mice.

Medicine

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer

Chenhao Hu^*1,2,3, Zhe Zhang^*1,2,3, Lei Zhang^1,2,3, Ruihan Liu^2,3, Jun Yan^1,2,3, Qi Sun^1,2,3, Guanghui Wang¹, Junjun She^1,2,3

¹Department of General Surgery, The First Affiliated Hospital of Xian Jiaotong University, ²Center for Gut Microbiome Research, Med-X Institute, The First Affiliated Hospital of Xian Jiao tong University, ³Department of High Talent, The First Affiliated Hospital of Xian Jiaotong University

The robotic technique described herein aims to detail a stepwise approach to robot-assisted total mesorectal excision and lateral pelvic lymph node dissection for locally advanced (T3/T4) rectal cancer located below the peritoneal reflection.

Medicine

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

Weijia Lu^*1, Ruimin Miao^*1, Sijun Hu¹, Junhong Liu¹, Fanqi Jin¹, Rui Xue Zhang¹

¹Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University

Detailed microsurgical techniques are demonstrated to establish a longer-term jugular vein cannulation rat model for sequential blood collection in the same animal. Physiological and hematological parameters have been monitored during the rat's recovery phase. This model has been applied to study pharmacokinetics of orally administered polyphenol without inducing animal stress.

Medicine

Orthopedic Robot-Assisted Femoral Neck System in the Treatment of Femoral Neck Fracture

Yuxuan Cong^*1, Pengfei Wen^*2, Yongchao Duan³, Hai Huang¹, Yan Zhuang¹, Pengfei Wang¹

¹Department of Orthopedic Trauma, HongHui Hospital, Xi'an Jiaotong University, ²Department of Joint Surgery, HongHui Hospital, Xi'an Jiaotong University, ³Department of Intraoperative Imaging, HongHui Hospital, Xi'an Jiaotong University

This article introduces a method of robot-assisted orthopedic surgery for screw placement during the treatment of femoral neck fracture using the femoral neck system, which allows for more accurate screw placement, improved surgical efficiency, and fewer complications.

Engineering

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics

Wenwen Yuan^1,2,3, Hang Yuan¹, Sixuan Duan^1,2, Ruiqi Yong¹, Jia Zhu^1,2,4, Eng Gee Lim^1,2, Ivona Mitrovic², Pengfei Song^1,2

¹School of Advanced Technology, Xi’an Jiaotong - Liverpool University, ²Department of Electrical Engineering and Electronics, University of Liverpool, ³State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, ⁴School of Intelligent Manufacturing and Smart Transportation, Suzhou City University

This protocol describes a straightforward process that utilizes convenient plastic micro-molds for simple microembossing operations to fabricate microchannels on nanofibrillated cellulose paper, achieving a minimum width of 200 µm.