Assessing Intestinal Permeability in Caenorhabditis elegans Using Smurf Staining

To begin, transfer 500 to 1, 000 L1 stage synchronized Caenorhabditis elegans worms on NGM agar plate and incubate at 20 degrees Celsius until the day of the test. Design the assay schedule for two experimental groups. Using M9 buffer, collect L4