Intracameral Injection of Hoechst to Assess the Bioavailability of Dye to the Corneal Endothelial Cell Layer

To begin, load five microliters of hoechst solution into a sterile 10 microliter glass Hamilton syringe, attached to a 34 gauge blunt needle. Insert this syringe needle into the anterior chamber through the self-sealing corneal tunnel, and inject