Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

1:08

Preparation of 35 mm Glass-bottom Culture Dishes For FRET-based Confocal Microscopy

3:18

Transient Transfection With Adenoviral Vector Carrying D1SR Ca²⁺ Indicator

4:17

Measurement of SR Luminal Ca²⁺ Using FRET-based Confocal Imaging

6:53

Measurement of Cytoplasmic Ca²⁺

7:55

Results: Distribution of the SR Ca²⁺ Indicator D1SR in Rat Aortic Smooth Muscle Cells

9:23

Conclusion