Selecting Females for Egg Collection via Interrupted Mating
1:31
Preparation of Sperm Solution
2:50
UV Treatment of Sperm Solution
3:37
Manual Extrusion of Mature Eggs
4:56
In Vitro Fertilization
5:51
Heat Shock Treatment
7:13
Selection of Embryos with a One-cycle Cytokinesis Stall
8:48
Results: Heat Shock 2 Treatment of Zebrafish Embryos
9:57
Conclusion
Transcription
The overall goal of this procedure is to promote diploidization in the early zebrafish embryo through inhibition of cytokinesis corresponding to one embryonic cell cycle. This method can help answer key questions in the field of genetics such as t
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A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.