Dilution Antigens and Generating Antigen Microarrays
3:21
Probe Antigen Microarrays with Diluted Sera
6:02
Scanning Antigen Microarrays and Exporting Data
7:40
Results: Detection of IgG and IgM Autoantibodies using Two-color Microarrays
9:29
Conclusion
Transcription
The overall goal of this technique is to rapidly screen for autoantibodies in a multiplex fashion. This method can help identify key questions in the field of autoimmunity, such as which autoantibodies are correlated with different disease states.
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We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.