Initial Screening of rPPEP-1 and Co-crystallization of rPPEP-1 with Ligands Using Commercial Screens
3:54
Crystal Optimization Using Microseeding
7:35
Crystal Mounting
10:45
Results: Evaluation of rPPEP-1 Crystal Growth Using Optimization Procedure
12:48
Conclusion
Transcription
The overall goal of this procedure is to efficiently grow single lattice diffraction quality crystals of recombinant proline proline endopeptidase-1, or rPPEP-1, protein. Without this method, rPPEP-1 instantly produces highly intergrown crystals n
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Proline-proline endopeptidase-1 (PPEP-1) is a secreted metalloprotease and promising drug-target from the human pathogen Clostridium difficile. Here we describe all methods necessary for the production and structure determination of this protein.