Isolate C. difficile from Different Environmental or Clinical Sources
3:46
Culturing C. difficile and Killing Kinetic Procedures
4:53
Prepare Samples for Scanning Electron Microscopy
6:26
Imaging C. difficile Cells on a Scanning Electron Microscope
9:21
Image Processing and Analysis
10:08
Results: Morphological Analysis of C. difficile in Response to Antibiotic Treatment
11:40
Conclusion
Transcription
The overall goal of this microbiological imaging method is to provide a more descriptive means to distinguish the phenotypic effects of pharmacological treatment. So this method can really help in the area of infective diseases. Specifically looki
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Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.