Determining Relative Cell-surface Protein Expression in Astrocytes by Biotinylation
4:40
Determining Internalization Rate of Cell-surface Proteins in Astrocytes by Biotinylation
8:52
Results: AQP4 Expression at the Cell-surface in the Presence of Laminin and AQP4 Internalization in Astrocytes
10:15
Conclusion
Transcription
In this video, two methods will be presented, cell-surface biotinylation, and pulse-chase biotinylation. In the following demonstration, we will be using astrocytes. These are the most abundant glial cell types in the brain, and they happen to exp
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Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.