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A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

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10:44 min

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June 2nd, 2019

DOI :

10.3791/58961-v

June 2nd, 2019

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5,644 Views

Cedar H. Baik¹, Mallory Geer¹, Olivia K. Travis², Denise C. Cornelius^1,2

¹Department of Emergency Medicine, University of Mississippi Medical Center, ²Department of Experimental Therapeutics and Pharmacology, University of Mississippi Medical Center

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Keywords Plate based Cytotoxicity Assay Rat Placental Natural Killer Cells Cytolytic Function Cytotoxic Activity Inexpensive Method Specialized Equipment Hazardous Materials CD8 Cytolytic Cells Target effector Cell Ratio Optimization Mononuclear Cell Isolation Placenta Homogenization Density Gradient Centrifugation Biotin labeled CD3 Antibody Magnetic Bead Separation

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