This protocol allows researchers to obtain distinct fractions of a cell without the use of specialized equipment, expensive reagents or complex techniques.The primary advantage of this protocol is the simplicity of the techniques which make it mod
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Here, we present a protocol to isolate the plasma membrane, cytoplasm and mitochondria of U937 cells without the use of high-speed centrifugation. This technique can be used to purify subcellular fractions for subsequent examination of protein localization via immunoblotting.