Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit
7:07
dCas9 and gRNA Transduction, Selection, and Clone Creation
10:18
Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA
12:08
Conclusion
Transcription
Noncoding RNAs often have multiple isoforms. In in vitro overexpression studies, it is often difficult to study all these expressed isoforms. This technique allows users to derive expression directly from the genome and enable the cells to express
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Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.