Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

6.8K Views

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09:01 min

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March 6th, 2021

DOI :

10.3791/62178-v

March 6th, 2021

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6,783 Views

Elizabeth J. Alessio¹, Dao-Qi Zhang¹

¹Eye Research Institute, Oakland University

Chapitres

0:04

Introduction

0:36

Tissue Preparation

3:04

Immunostaining and Refractive Index Matching

4:05

Mounting

5:30

Imaging

6:36

Results: Improved 3D Resolution of Fine Processes of Retina by Modified CLARITY Tissue Clearing Method

8:39

Conclusion

Transcription

This protocol allows for the investigation of the fine morphology of retinal neurons and can give insight into the cellular and subcellular morphological changes that occur in disease states. This method significantly improves the optical transpar

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Résumé

Here we present a protocol to adapt the CLARITY method of the brain tissues for whole-mount retinas to improve the quality of standard immunohistochemical staining and high-resolution imaging of retinal neurons and their subcellular structures.

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Keywords Immunostaining

Whole mount Retina

CLARITY Tissue Clearing

Subcellular Structures

Enucleation

Cornea