Results: Planarian Ovary Ultrastructural Analysis and Antibody Staining
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Conclusion
Transcription
The goal of this method is to dissect the ovaries of the planarian Schmidtea mediterranea for cellular and subcellular analysis of oocyte biology with TEM or antibody staining. This protocol uses a brief fixation step to facilitate the localizatio
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This protocol presents steps taken to dissect ovaries in the freshwater planarians, Schmidtea mediterranea. The dissected ovaries are compatible for antibody immunostaining and ultrastructural analysis with transmission electron microscopy to study the cell biology of the oocytes and somatic cells, providing an imaging depth and quality that were previously inaccessible.