Prepare the Inoculum and Inoculate the Plant Roots
5:00
Determining Race by Infested Kernel Method (IKM)
7:04
Combine Soil and Grain
8:04
Determining Race by Modified Tray-Dip Method (MTDM) and Disease Rating
10:08
Results: Phenotypic Results of Race Identification Methods
10:56
Conclusion
Transcription
This protocol can help those involved in the watermelon community evaluate the fusarium wilt pathogen, and this will help determine the pathogen populations that are present and help manage it effectively. Race-typing fusarium wilt is complex and
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Managing Fusarium wilt of watermelon requires knowledge of the pathogen races present. Here, we describe the root-dip, infested kernel seeding, and modified tray-dip inoculation methods to demonstrate their efficacy in race-typing of the pathogenic fungus Fusarium oxysporum f. sp niveum (Fon).