Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-¹³C]Pyruvate and ¹³C/³¹P NMR Spectroscopy

1:17

Krebs-Henseleit Buffer Preparation

1:45

Perfusion System Preparation

2:29

Calibration and Preparation of the NMR Spectrometer for Acquisition

3:22

Animal Preparation, Surgical Procedure, and Perfusion of the Heart in the NMR Tube

9:23

Acquiring Data for Cardiac Energetics and pH

10:09

DNP Spin Polarization and Dissolution

11:25

Hyperpolarized ¹³C Spectroscopy

12:07

Representative Results

14:40

Conclusion