Determination of Sex Ratio in the Pupae and Larvae Viability
5:32
Determination of Larvae-to-Pupae Development Time and Male Contribution
6:56
Determination of Mosquito Longevity
7:25
Results: Measurement of Development and Survival Time in Transgenic Aedes aegypti Mosquitoes
9:01
Conclusion
Transcription
This protocol demonstrates standard measurements, reflective of fitness, in aedes aegypti mosquitoes, these fitness parameters were chosen because they reflect reproductive success, are simple to measure, and are commonly reported in the literatur
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The present protocol describes how to measure common life parameter data in Aedes aegypti mosquitoes, including fecundity, wing size, fertility, sex ratio, viability, development times, male contribution, and adult longevity. These measurements can be used to assess the fitness of transgenic mosquitoes.