This study is supported by the grant of National Social Science Foundation (22FYSB023), Hubei Industrial Design Center Research Foundation (08hqt201412046), and Humanities and Social Science Foundation of Hubei Provincial Education Department (15Y054).

Full written informed consent was provided by all participants. Ethics approval was not applicable since no animal or human tissue samples were included in the present study.
1. Investigating the requirements of the user community
<ol>
	<li>Deliver questionnaires to the biologists/experimenter working on cell wound healing assays. Collect the completed questionnaires and use them for the study. For this study, from 12 Oct 2021 to 3 Feb 2022, 100 questionnaires were delivered...

Innovating an Improved Cell Scraper for Cell Wounding by User Requirements Analysis

The present study aimed to develop an automatic mechanized tool for cell wound healing assay. To the best of our knowledge, it represents the first attempt to apply a mechanized driven structure to create cell wounds in a one-click way automatically. Through this, we aim to address the shortcomings of the traditional tips-based method, such as low reproducibility and unstable scratch state. Benefiting from the positive results and the encouraging feedback from the user community, the cell scraper is expected to provide e...

Tumors are characterized by distinct hallmarks such as selective growth advantages, metabolic rewiring, and immune modulation, all of which intriguingly contribute to enhanced cell migration, a critical malignant behavior of tumor cells. This feature directly affects the distant metastasis of the primary tumor, compromising the long-term survival of patients1,2,3. Selective growth advantages enable cancer cells to outcompete normal cells, while metabolic rewiring supports this rapid proliferation by altering energy pathways. Concurrently, immune modulation allows tumors to evade the body&#39;s defenses. Studies underscore the severity of this issue, showing that lung metastasis, often a result of enhanced cell migration, is a terminal event leading to the death of patients with various cancers4. For instance, breast cancers5, cervical carcinoma4, and osteosarcoma6 account for 20%, 9%, and 30% of such cases, respectively. Therefore, assessing tumor cell migration has become integral to current oncology research, further highlighting the multifaceted nature of tumor progression.
Cell wound healing assay is an easy-to-use method for measuring in vitro cellular migration, often employed in oncological studies7. Most experimenters use pipette tips to create cell wounds manually8. Although such a method could form cell wounds rapidly and conveniently, it still has many limitations that affect reproducibility and accuracy for evaluating cell migration. Firstly, using pipette tips to create scratches manually is highly influenced by the operator&#39;s operating angle, force, and speed, affecting the method&#39;s repeatability8. Secondly, tip-generated cell defects usually have jagged edges rather than straight edges because pipette tips are plastic products with certain elasticity9. Some studies generate wounds by placing prefabricated culture inserts directly into the cell culture plate to restrict the range of cell proliferation10. This approach circumvents the limitation of the tips-based method, such as jagged edges and reproducibility. However, even with biocompatible materials, the long-term coexistence of the embedding with the cells still impacts cell growth11. Moreover, the embedding may also cause cellular epigenetic changes in the marginal zone due to contact restriction12. Also, contact-insert produced from biocompatible materials are expensive and difficult to reuse, limiting their feasibility13. Therefore, a novel, reproducible, and practical tool is needed to easily quantify in vitro cellular migration.
The primary goal of this method is to introduce an innovative tool for quantifying in vitro cellular migration in oncological studies, addressing the limitations of existing techniques and enhancing reproducibility and accuracy in assessing cellular migration.
The rationale behind this technique&#39;s development lies in the critical importance of evaluating tumor cell migration in oncology. Tumors exhibit distinctive hallmarks, including selective growth advantages, metabolic rewiring, and immune modulation, all contributing to enhanced cell migration, a fundamental aspect of cancer malignancy. This method aims to provide a more dependable means of studying cellular migration, contributing to a deeper understanding of tumor behavior.
This method offers substantial advantages over existing techniques. Manual scratch assays can suffer from operator-dependent interference, while culture inserts may impact cell growth and gene expression. In contrast, this method offers improved repeatability, accuracy, and practicality, presenting a cost-effective solution for measuring in vitro cellular migration in oncology research. It addresses a crucial need for a reliable and accessible tool to study cell migration in various cancer types, making it a valuable addition to the field.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>CCK-8 Kit</td>
			<td>Beyotime Company, China</td>
			<td>C0037</td>
			<td></td>
		</tr>
		<tr>
			<td>digital microscope system</td>
			<td>Olympus</td>
			<td>IX81</td>
			<td></td>
		</tr>
		<tr>
			<td>fetal bovine serum</td>
			<td>Gibco, USA</td>
			<td>16000044</td>
			<td></td>
		</tr>
		<tr>
			<td>HOS&#160;</td>
			<td>Procell Life Science &#38; Technology Co., Ltd</td>
			<td>CL-0360</td>
			<td></td>
		</tr>
		<tr>
			<td>Image-Pro Plus</td>
			<td>Media Cybernetics</td>
			<td>version 6.0</td>
			<td></td>
		</tr>
		<tr>
			<td>KeyShot</td>
			<td>Luxion</td>
			<td>version 11.0</td>
			<td>3D rendering software</td>
		</tr>
		<tr>
			<td>microplate reader</td>
			<td>BioTek, German</td>
			<td>ELX808</td>
			<td></td>
		</tr>
		<tr>
			<td>Minimum Essential Medium</td>
			<td>Gibco, USA</td>
			<td>11095080</td>
			<td></td>
		</tr>
		<tr>
			<td>Pantone matching system</td>
			<td>Pantone</td>
			<td></td>
			<td>commercial color matching</td>
		</tr>
		<tr>
			<td>penicillin-streptomycin</td>
			<td>Beyotime Company, China</td>
			<td>ST488</td>
			<td></td>
		</tr>
		<tr>
			<td>Photoshop</td>
			<td>Adobe</td>
			<td></td>
			<td>photo and design software</td>
		</tr>
		<tr>
			<td>Rhinoceros 3D</td>
			<td>Robert McNeel &#38; Associates</td>
			<td>version 7.0</td>
			<td>3D design software</td>
		</tr>
		<tr>
			<td>TC20 Automated Cell Counter</td>
			<td>Bio-Rad</td>
			<td>TC20</td>
			<td></td>
		</tr>
		<tr>
			<td>Trypsin</td>
			<td>Cytiva&#160;HyClone, United State</td>
			<td>SH30042.01</td>
			<td></td>
		</tr>
	</tbody>
</table>

a tool to automatically create stable and reproducible cell-free gaps for improving the reliability of cell wound healing assay

The reliability of cellular migration measurement in wound healing assays is frequently undermined by the prevalent methodological instability, i.e., tip-based method. We introduce an innovative instrument designed to address these limitations. Our novel cell scraper surpasses the current approach, generating a more consistent and stable cell-free gap. Repeated biological experiments reveal that the cell-free gap produced by the cell scraper exhibits straighter edges and uniform size and shape compared to the tip-based technique (p &lt; 0.05). In terms of product design, the cell scraper boasts a refined color scheme suited for laboratory environments, enhancing the monitoring of experimental outcomes, and permits sterilization through autoclaving for reuse. Notably, after treatment, the cell scraper demonstrates a negligible effect on cell viability and proliferation (97.31% and 24.41%, respectively). Conversely, the tip-based method yields lower cell viability (91.37%) and proliferation (18.79%). This investigation presents the cell scraper as a novel, reusable device capable of generating reproducible cell-free gaps while preserving cellular viability, thereby augmenting the reliability of wound healing assays in comparison to existing techniques.

Author Spotlight: Designing an Innovative Cell Scraper Using Interaction Experience Design and User Requirement Analysis

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay

Through the incorporation of interaction experience design and user requirements analysis, we introduce a innovative cell scraper that enhance cellular wound healing assays in terms of reproducibility, dependability, practicality, cellular integrity, and user experiences. We authorized consultative rhinal, keystone, and photocell for product visualizations. This approach that only is in pre-producing the actual product, but also we enhance communications among team members.Additionally, it contributes to reducing the overall year costs. In experimental validations, the cell scraper showed higher survival and proliferation risk than the TIPS group post wound completion confirmed by an EdU assay. This indicates a scraper superiority in creating stable biologically reproducible cell walls compared to the traditional method with minimal impact on cell health.

Dissecting user demands for tools to generate cell wounds 
The current experimental method to generate cell wounds demands further enhancement to address many issues that compromise biological reproducibility, robustness, economic consumption, and user experience of cell wound healing assay. We utilized the hard laddering method to analyze the requirements of users involved in biological experiments via questionnaires29 (Figure 1A). The informati...

Through the incorporation of interaction experience design and user requirements analysis, we introduce an innovative cell scraper that enhances cellular wound healing assays in terms of reproducibility, dependability, practicality, cellular integrity, and user experience.

The reliability of cellular migration measurement in wound healing assays is frequently undermined by the prevalent methodological instability, i.e., ...

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JoVE Journal

Biology

238 vues.  Kunming University of Science and Technology. Grâce à l’intégration de la conception de l’expérience d’interaction et de l’analyse des exigences de l’utilisateur, nous introduisons un grattoir cellulaire innovant qui améliore les tests de cicatrisation cellulaire en termes de reproductibilité, de fiabilité, de praticité, d’intégrité cellulaire et d’expérience utilisateur. Nous avons autorisé la consultation rhinale, trapézoïdale et photoélectrique pour les visualisations de p...

Vidéo: Pleins feux sur l’auteur : Conception d’un racleur de cellules innovant à l’aide de la conception de l’expérience d’interaction et de l’analyse des besoins de l’ut