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universidade federal de são paulo

2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue
Bruna A. G. de Melo 1, Elisa M. Cruz 1, Taís N. Ribeiro 1, Mayara V. Mundim 1, Marimelia A. Porcionatto 1
1Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo

Here we report a method of 3D bioprinting murine cortical astrocytes for biofabricating neural-like tissues to study the functionality of astrocytes in the central nervous system and the mechanisms involving glial cells in neurological diseases and treatments.

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Immunology and Infection

Demystifying In Vivo Bioluminescence Imaging of a Chagas Disease Mouse Model for Drug Efficacy Studies
Adriana C. da Silva 1, Jadel M. Kratz 2, Priscylla G. M. Morgado 1,3, Lucio H. Freitas-Junior 1, Carolina B. Moraes 1,4
1Departamento de Microbiologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo, 2Drugs for Neglected Diseases initiative (DNDi) Latin America, 3Programa de Pós-Graduação em Microbiologia e Imunologia, Universidade Federal de São Paulo, 4Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo

The protocol provided here describes the detailed steps to perform drug efficacy studies in a bioluminescent model of Trypanosoma cruzi infection, with a focus on data acquisition, analysis, and interpretation. Troubleshooting and quality control procedures to minimize technical issues are also provided.

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