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Université de Bourgogne Franche-Comté

1 ARTICLES PUBLISHED IN JoVE

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Biology

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Geetika Raizada 1, Balasubramaniam Namasivayam 2, Sameh Obeid 3, Benjamin Brunel 1, Wilfrid Boireau 1, Eric Lesniewska 4, Celine Elie-Caille 1
1Université de Franche-Comté, CNRS UMR-6174, FEMTO-ST Institute, 2Lille Neuroscience & Cognition research centre, 3Institut Galien Paris-Saclay, CNRS UMR-8612 , Université Paris-Saclay, 4Interdisciplinary Lab Carnot Bourgogne LICB, CNRS UMR-6303, Université de Bourgogne Franche-Comté

This paper proposes a new generation of multiparametric analytical platforms with increased throughput for the characterization of extracellular vesicle subsets. The method is based on a combination of multiplexed biosensing methods with metrological and morphomechanical analyses by atomic force microscopy, coupled with Raman spectroscopy, to qualify vesicular targets trapped on a microarray biochip.

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