Université Côte d'Azur, Hôpital Pasteur, CHU de Nice

1 ARTICLES PUBLISHED IN JoVE

Biology

Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

Christophe Bontoux^1,2,3,4,5, Virginie Lespinet-Fabre^1,2,3,4, Olivier Bordone^1,2,3,4, Virginie Tanga^1,2,3,4, Maryline Allegra^1,2,3,4, Myriam Salah^1,2,3,4, Salomé Lalvée^1,2,3,4, Samantha Goffinet^1,2,3,4, Jonathan Benzaquen^3,4,5,6, Charles-Hugo Marquette^3,4,5,6, Marius Ilié^1,2,3,4,5, Véronique Hofman^1,2,3,4,5, Paul Hofman^1,2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025), Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA, Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge, Université Côte d'Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne, Université Côte d'Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice, Université Côte d'Azur

The increase of molecular biomarkers to be tested for non-squamous non-small cell lung cancer (NS-NSCLC) care management has prompted the development of fast and reliable molecular detection methods. We describe a workflow for genomic alteration assessment for NS-NSCLC patients using an ultra-fast-next generation sequencing (NGS) approach.