Using Avian Skin Explants to Study Tissue Patterning and OrganogenesisTingxin Jiang 1, Maeve Secor 2, Rusty Lansford 3, Randall B. Widelitz 1, Cheng Ming Chuong 1
1Department of Pathology, Keck School of Medicine of University of Southern California, 2Molecular and Computational Biology, University of Southern California, 3Department of Radiology, Keck School of Medicine of University of Southern California
Here we describe protocols for three types of avian embryonic skin explant cultures that can be used to examine tissue interactions, 4D imaging timelapse movie (3D plus time), global or local perturbation of molecular function, and systems biology characterization.