National Institute of Biology

2 ARTICLES PUBLISHED IN JoVE

Biology

Studying Cell Death Initiation Using a Digital Microscope

Tina Arnšek^*1, Nuša Golob^*1, Nastja Marondini¹, Maruša Pompe-Novak^1,2, Kristina Gruden¹, Tjaša Lukan¹

¹National Institute of Biology, ²University of Nova Gorica

Here we present a protocol for studying the rate of programmed cell death initiation by continuously imaging inoculated leaves following cell death induction.

Biology

High Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato

Lamis Osama Anwar Abdelhakim¹, Barbora Pleskačová¹, Natalia Yaneth Rodriguez-Granados², Rashmi Sasidharan², Lucia Sandra Perez-Borroto³, Sophia Sonnewald⁴, Kristina Gruden⁵, Ute C. Vothknecht⁶, Markus Teige⁷, Klára Panzarová¹

¹PSI (Photon Systems Instruments), spol. s r.o. Drasov, ²Plant Stress Resilience, Institute of Environmental Biology, Utrecht University, ³Plant Breeding, Wageningen University and Research, ⁴Department of Biology, Biochemistry, Friedrich-Alexander Universität Erlangen-Nürnberg, ⁵Department of Biotechnology and Systems Biology, National Institute of Biology, ⁶Plant Cell Biology, Institute of Cellular and Molecular Botany, University of Bonn, ⁷Department of Functional & Evolutionary Ecology, University of Vienna

We designed an image-based phenotyping protocol to determine the morphological and physiological responses to single and combined heat, drought, and waterlogging treatments. This approach enabled the identification of early, late, and recovery responses at a whole plant level, particularly above-ground parts, and highlighted the necessity of using multiple imaging sensors.