Sign In

Kochi University of Technology

1 ARTICLES PUBLISHED IN JoVE

image

Biology

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay
Yuna Satake 1, Nozomi Gotouda 1, Seiji Tanaka 1,2
1Graduate School of Engineering, Kochi University of Technology, 2School of Engineering Science, Kochi University of Technology

The interactions of biomolecules, such as protein-protein interactions, are the molecular basis of biological functions. If interaction-null/impaired mutants that specifically lack the relevant interaction can be isolated, they will greatly help to understand the function(s) of this interaction. This article presents an efficient way to isolate interaction-null/impaired mutants.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved