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Regeneron Pharmaceuticals Inc.

1 ARTICLES PUBLISHED IN JoVE

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Chemistry

Host Cell Protein Analysis using Enrichment Beads Coupled with Limited Digestion
Sisi Zhang 1, Hui Xiao 1, Ning Li 1
1Analytical Chemistry, Regeneron Pharmaceuticals Inc.

A protocol is presented for enriching host cell proteins (HCPs) from drug products (DP) and detecting peptides using proteome enrichment beads. The method is demonstrated using an in-house manufactured monoclonal antibody (mAb) drug substance (DS), which is a well-characterized reference material for evaluating and comparing different methods in terms of performance.

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