JoVE Logo

Sign In

University of Neuchatel

2 ARTICLES PUBLISHED IN JoVE

image

Environment

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Tina Wunderlin 1, Thomas Junier 2, Christophe Paul 3, Nicole Jeanneret 3, Pilar Junier 3
1Department of Biological Sciences, Macquarie University, 2Vital-IT group, Swiss Institute of Bioinformatics, 3Laboratory of Microbiology, University of Neuchatel

A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.

image

JoVE Journal

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Venkatasalam Shanmugabalaji 1, Véronique Douet 1, Birgit Agne 2, Felix Kessler 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved