UC Irvine

3 ARTICLES PUBLISHED IN JoVE

Biology

Laser Capture Microdissection of Mammalian Tissue

Robert A Edwards¹

¹Department of Pathology, University of California, Irvine (UCI)

Laser Capture Microdissection of Mammalian Tissue

Biology

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Hassan Khalil¹, Wenxian Nie¹, Robert A Edwards², James Yoo¹

¹Department of Surgery, UCLA, ²Department of Pathology, UC Irvine

The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.

Bioengineering

Zebra II as A Novel System to Record Electrophysiological Signals in Zebrafish

Ramses Seferino Trigo Torres¹, Mao-Hsiang Huang², Mohamed Benomar², Tai Le¹, Tim Etchells³, Xiaolei Xu⁴, Michael P. H. Lau³, Hung Cao^1,2

¹Department of Biomedical Engineering, UC Irvine, ²Department of Electrical Engineering and Computer Science, UC Irvine, ³Sensoriis Inc., ⁴Department of Biochemistry and Molecular Biology, Mayo Clinic

The study introduces Zebra II, an advanced system for prolonged ECG acquisition and analysis in zebrafish. This system features an independent perfusion system and multiple-point electrodes for handling multiple fish in controlled environments. Acute effects of Amiodarone were assessed and analyzed with wild-type zebrafish.