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University of Lyon 1

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
Céline Delloye-Bourgeois *1, Arnaud Jacquier *1, Julien Falk 1, Valérie Castellani 1
1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534, University of Lyon

We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

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Neuroscience

Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
Camille Charoy 1, Elise Arbeille 1, Karine Thoinet 1, Valérie Castellani 1
1CGphiMC, UMR CNRS 5534, University of Lyon 1

The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.

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