Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensisJesse Q. Nguyen 1, Xhavit Zogaj 1, Aanuoluwa A. Adelani 1, Ping Chu 2, Jieh-Juen Yu 1, Bernard P. Arulanandam 1, Karl E. Klose 1
1South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio, 2Midwestern University
This protocol describes intratracheal inoculations of Fischer 344 rats with Francisella tularensis. This procedure mimics pulmonary exposure of humans to this potential biothreat agent and can be used to test vaccine and therapeutic efficacy against pulmonary tularemia.