Co-Culture In Vitro Systems to Reproduce the Cancer-Immunity CycleNicoletta Manduca *1, Ester Maccafeo *1, Adele De Ninno 2, Antonella Sistigu 1,3, Martina Musella 1
1Dipartimento di Medicina e Chirurgia Traslazionale, Università Cattolica del Sacro Cuore, 2CNR Institute for Photonics and Nanotechnology, 3Fondazione Policlinico Universitario ‘A. Gemelli’ - IRCCS
Here, we detail a simple, fast, and reliable multiparametric flow cytometry- and tumor-on-a-chip-assisted protocol to monitor and characterize the steps constituting the cancer-immunity cycle. Indeed, a thorough understanding of the interplays between cancer and immune cells provides critical insights to outsmart tumors and guide clinical care.